Chromatin immunoprecipitation of Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-2/DNA complexes were immunoprecipitated using 5 µg Sheep Anti-human GLI-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47500) or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The bcl-2 promoter was detected by standard PCR.